ISO 19574 pdf download – Footwear and footwear components 一Qualitative test method to assess antifungal activity (growth test).
Do not blend Trichophyton mentagrophytes suspension with any other micro-fungi suspension. 10 Preparation of test specimens
10.1 General
Test only the components or materials that are claimed to be antifungal. If the whole footwear is claimed to be antifungal, major components, including upper, lining, insole, insock. outsole shall be tested separately.
In the case where only one material of a component is claimed to be antifungal, it shall be tested separately, If possible. Otherwise, the whole component shall be tested.
each test specimen shall be at least 80 % or the surFace area of the component or material, It a single material accounts for less than 80 %, take the two main materials used In the composition of the component.
The test specimens can be obtained directly from the footwear or from components and materials prior to construction.
10.2 Test and control specimens
The area of test specimens should be about I 60() mm2 for testing, and have a thickness of less than 5,0 mm. The area and the weight shall he reported in the test report, If a larger test specimen is used, then the volume of micro.fungal suspension should be increased proportionally.
If it is not possible to lower the thickness of the test specimens (for example, components are thicker and can’t be separated or cut without changing critical properties, such surface morphology, which can affect how the fungi interact with the surface), the thickness shall be indicated in the test report.
Use material identical to the test material but without antifungal treatment as control specimens. If no such material is available, sterilized filter paper can be used as control specimens.
At least three test specimens shall be taken for each material or component and three control specimens for each test.
10.3 Pre-treatment of test and control specimens
Pre-treatment of test and control specimens is optional and should only be conducted if necessary due to high bioburden (contamination etc.).
If sterilization methods are applied, they shall be reported in detail, and shall not affect the antifungal properties or the material Itself.
In order to increase the accuracy of the assessment, a grid (6.17) shall be placed over the test specimento enable percentage of surface growth to be assessed.
lf the visual assessment of the test specimens of one batch varies over a range of two scale ratings, thetest shall be repeated.
lt has been found that colour photographs are useful records of the visual examination and shouldbe included in the final report wherever possible. But depending on the kind and colour of the testspecimen, it is not always clear what is seen on them and for example sporulating Aspergillus on a blackvelour leather is hard to recognize on photos, so photos should be included in the test report. If needed,such images can be made available for clarification even outside the context of a test report.
lt might be helpful to record if the observed growth is due to mycelia with or without formation ofconidiophores, i.e. sporulation. For the evaluation, all kinds of growth shall be taken into account,without consideration of absence or presence of sporulation.
13 Test report
The test report shall include at least the following information:
a) a reference to this document, i.e. lS0 19574:2022;
b) all information necessary for the complete identification of the treated test components,e.g.
positions,areas, weights;
c) preparation of test specimen，including pre-treatment methods,if applied (i.e. sterilization
method);
d) the species, serial number of test strains for different materials;
e)the volume of the inoculum on each specimen;
f)surfactant (wetting agent) and its concentration added into the test inoculums.
ISO 19574 pdf download – Footwear and footwear components 一Qualitative test method to assess antifungal activity (growth test)
